Fourier-Transform Midinfrared Spectroscopy for Analysis and Screening of Liquid Protein Formulations
نویسنده
چکیده
LEVEL: ADVANCED I n the first half of this two-part article (1), we introduced the physical principle behind infrared (IR) absorptions and experimental setups used for investigating protein samples. This second part concludes by focusing on the use of IR spectroscopic data in formulation stability studies and for characterizing protein secondary structures and related changes (e.g., resulting from protein–excipient interactions). We also examine IR’s use in screening protein formulations.
منابع مشابه
سنتز و بررسی رفتار آنالیز حرارتی مایع یونی پرانرژی آمینوگوانیدینیوم: گوانیدینیوم تری فلوئورواستات
Aminoguanidinium: guanidinium trifluoroacetate (AG:GTFA) has recently been considered in the defense industries as an insensitive and energetic ionic liquid for using in melt-pour explosive formulations. In the first section of this research, aminoguanidinium trifluoroacetate (AGTFA) with efficiency of %65 and melting point of 58.0±2.0 °C was synthesized by hydrogenation of nitroguan...
متن کاملTheophylline-Ethylcellulose Microparticles: Screening of the Process and Formulation Variables for Preparation of Sustained Release Particles
Objective(s) The aim of this study was to formulate and evaluate microencapsulated controlled release preparations of theophylline using ethylcellulose as the retardant material with high entrapment efficiency. Materials and Methods Microspheres were prepared by water-in-oil-in-oil (W/O1/O2) emulsion-solvent diffusion (ESD). A mixed solvent system consisting of acetonitrile and dichloromet...
متن کاملIdentification and Quantification of Texture Soy Protein in A Mixture with Beef Meat Using ATR-FTIR Spectroscopy in Combination with Chemometric Methods
Meat, as an important source of protein, is one of the main parts of many people’s diet. Due toeconomic interests and thereupon adulteration, there are special concerns on its accurate labeling.In this study Fourier transform infrared (ATR-FTIR) spectroscopy combined with chemometrictechniques (principal component analysis (PCA), artificial neural networks (ANNs), and partial<...
متن کاملLactic-based Novel Amine Ionic Liquid: Synthesis and Characterization of [DEA][Lac]
In this study, a novel amine ionic liquid “Diethanolamine Lactic” [DEA][Lac] was synthesized. The replacement of halogenated ion fluid was used as a modified methodology for the preparation of diethanolamine based on lactic acid. The ionic liquid was characterized using the Fourier transform infrared (FTIR) spectra and the nuclear magnetic resonance (NMR) spectroscopy. The changes in bands' wav...
متن کامل